The Cox IV Rabbit Monoclonal Antibody is a high-affinity antibody designed for research involving Cox IV, a critical subunit of cytochrome c oxidase and a key player in the electron transport chain. The antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in various applications such as Western blot, immunohistochemistry, and immunofluorescence.Cox IV is essential for mitochondrial function and plays a crucial role in cellular respiration, making it a valuable target for studies in bioenergetics, metabolism, and mitochondrial disorders. By targeting Cox IV, researchers can gain insights into mitochondrial dysfunction, oxidative stress, and metabolic diseases, providing potential avenues for therapeutic intervention and drug development.
The Cox IV Rabbit Monoclonal Antibody enables precise detection and quantification of Cox IV protein levels in different cell types and tissue samples, allowing for detailed analysis of mitochondrial function and energy production. Its high specificity and sensitivity make it a reliable tool for investigating the role of Cox IV in health and disease, advancing our understanding of mitochondrial biology and potential treatment strategies.
Product Name:
COX IV Rabbit Monoclonal Antibody
SKU:
CAB11631
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COX IV (P13073).
COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1; COX IV
Positive Sample:
HeLa,MCF7,Mouse brain,Mouse heart,Rat heart
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion inner membrane.
Calculated MW:
20kDa
Observed MW:
17kDa
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1327
Clone Number:
ARC2518
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using COX IV Rabbit mAb (CAB11631) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
