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COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1731)

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SKU:
SBRS1731
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Indirect
€999

Description

COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1731)

Product SKU:SBRS1731
Size:96T
Gene Names:IGG
Target Species:Human
Compatible Sample Types:Dried Blood, Plasma, Saliva, Serum
Design Principle:Indirect
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
  1. SARS-CoV-2 N-protein coated 96-well microplate
  2. Wash Buffer
  3. Positive Control
  4. Biotinylated Anti-Human IgG
  5. HRP-Streptavidin concentrate
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Assay Diluent
  9. 5X Sample Diluent
  10. 1X PBS (DBS version)
  11. Tween-20 (DBS version)
  12. Protease Inhibitor Cocktail (DBS version)

Other materials and equipment required:

The Assay Genie COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1731) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Microplate reader capable of measuring absorbance at 450 nm
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Distilled or deionized water
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 ul positive control or sample to each well. Incubate 1 hour at room temperature.
  3. Add 100 µl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
  4. Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
  5. Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
  6. Add 50 ul Stop Solution to each well. Read at 450 nm immediately.

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