The COPB2 Monoclonal Antibody (CAB21294) is a high-quality antibody developed for reliable detection and analysis of target proteins. The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
COPB2 Monoclonal Antibody
SKU:
CAB21294
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC52626
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:3000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OPDD, MCPH19, beta'-COP, COPB2
Positive Sample:
Mouse testis, HeLa, MCF7, Jurkat
Cellular Localization:
Cytoplasm, Golgi Apparatus Membrane .
Calculated MW:
102kDa
Observed MW:
102kDa
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.
Purification Method
Affinity purification
Gene ID
9276
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COPB2 Rabbit mAb (CAB21294) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
