COMT Rabbit Monoclonal Antibody (CAB4435)
- SKU:
- CAB4435
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- HEL-S-98n
- Research Area:
- Metabolism
Description
| Product Name: | COMT Rabbit Monoclonal Antibody |
| SKU: | CAB4435 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COMT (P21964). |
| Sequence: | MPEA PPLL LAAV LLGL VLLV VLLL LLRH WGWG LCLI GWNE FILQ PIHN LLMG DTKE QRIL NHVL QHAE PGNA QSVL EAID TYCE QKEW AMNV GDKK GKIV |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | HEL-S-98n; COMT |
| Positive Sample: | HepG2,Raji,U-87MG,Rat liver,Rat kidney |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein. |
| Calculated MW: | 30kDa |
| Observed MW: | 25kDa/28kDa |
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
| Purification Method: | Affinity purification |
| Gene ID: | 1312 |
| Clone Number: | ARC1006 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (CAB4435) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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