The COMT Rabbit Monoclonal Antibody (CAB4435) is a valuable tool for researchers studying the COMT protein, an enzyme involved in the breakdown of dopamine and other neurotransmitters. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.By binding specifically to the COMT protein, this antibody enables the detection and analysis of COMT in various cell types, making it ideal for investigations in neuroscience, psychiatry, and pharmacology.
The role of COMT in the regulation of neurotransmission and behavior makes it a key target for studies related to neurological disorders, addiction, and mental health.Understanding the function and regulation of COMT is critical for developing new treatments and medications that target the dopamine pathway and neurotransmitter signaling. The COMT Rabbit Monoclonal Antibody provides researchers with a reliable tool for exploring the complex mechanisms underlying neurotransmitter metabolism and behavior.
Product Name:
COMT Rabbit Monoclonal Antibody
SKU:
CAB4435
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COMT (P21964).
Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein.
Calculated MW:
30kDa
Observed MW:
25kDa/28kDa
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Purification Method:
Affinity purification
Gene ID:
1312
Clone Number:
ARC1006
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (CAB4435) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
