The COMT Polyclonal Antibody (CAB1294) is specifically designed for researchers studying catechol-O-methyltransferase (COMT), an enzyme involved in the breakdown of neurotransmitters like dopamine, epinephrine, and norepinephrine. This polyclonal antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the COMT protein, this antibody enables accurate detection and analysis in various cell types.COMT is known to play a critical role in the regulation of neurotransmitter levels in the brain, impacting various physiological and psychological functions.
Dysregulation of COMT activity has been implicated in neurological disorders such as Parkinson's disease and schizophrenia, making it a promising target for therapeutic interventions.Researchers in the fields of neuroscience, pharmacology, and psychiatry can benefit from using the COMT Polyclonal Antibody to further understand the mechanisms underlying these disorders and develop novel treatment strategies. Its high specificity and sensitivity make it an invaluable tool for investigating the role of COMT in health and disease.
Product Name:
COMT Rabbit Polyclonal Antibody
SKU:
CAB1294
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HEL-S-98n; COMT
Positive Sample:
MCF-7,HT-29,THP-1,A-431,HepG2,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein.
Calculated MW:
30kDa
Observed MW:
25kDa, 30kDa
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Purification Method:
Affinity purification
Gene ID:
1312
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using COMT Rabbit pAb (CAB1294) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
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