The COL8A2 Polyclonal Antibody (PAC020976) is a valuable tool for researchers studying the COL8A2 protein, which is known to be involved in the formation and maintenance of collagen fibrils in the cornea and other tissues. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the COL8A2 protein, this antibody enables precise detection and analysis of COL8A2 in a variety of cell types, making it ideal for investigations in the fields of ophthalmology and connective tissue disorders.
COL8A2, a critical component of type VIII collagen, plays a crucial role in maintaining the structural integrity of the cornea and other tissues in the body. Research on COL8A2 is essential for understanding the pathology of diseases such as Fuchs dystrophy and early-onset corneal endothelial dystrophy, which are characterized by abnormalities in collagen fibril organization. By elucidating the functions of COL8A2, researchers may uncover new insights into the mechanisms underlying these conditions and pave the way for the development of targeted therapeutic strategies.
Antibody Name:
COL8A2 Antibody (PACO20976)
Antibody SKU:
PACO20976
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human COL8A2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20976(COL8A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO20976(COL8A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes the α 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with α 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants.
Synonyms:
collagen, type VIII, α 2
UniProt Protein Function:
COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.Protein type: Secreted, signal peptide; Secreted; Extracellular matrixChromosomal Location of Human Ortholog: 1p34.2Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular regionBiological Process: collagen catabolic process; extracellular matrix organization and biogenesisDisease: Corneal Dystrophy, Fuchs Endothelial, 1; Corneal Dystrophy, Posterior Polymorphous, 2
UniProt Protein Details:
NCBI Summary:
This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
