The COL5A3 Polyclonal Antibody (PAC023314) is a valuable tool for researchers studying collagen type V alpha 3 chain (COL5A3), a crucial component of the extracellular matrix. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the COL5A3 protein, researchers can accurately detect and analyze this important collagen molecule in various cell types, making it an ideal choice for studies in connective tissue disorders, wound healing, and fibrosis research.COL5A3 is essential for maintaining the structural integrity of tissues and plays a key role in collagen fibril formation. Dysregulation of COL5A3 has been linked to various pathological conditions, including Ehlers-Danlos syndrome and other connective tissue disorders.
By understanding the function and expression of COL5A3, researchers can gain insights into the mechanisms underlying these diseases and potentially develop targeted therapies to alleviate their symptoms.In summary, the COL5A3 Polyclonal Antibody is a valuable tool for researchers interested in investigating the role of COL5A3 in health and disease. Its high reactivity and specificity make it ideal for a wide range of experimental applications, providing valuable insights into the biology of collagen type V alpha 3 chain and its implications for human health.
Synthesized peptide derived from internal of human Collagen V alpha 3.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Collagen V alpha 3 antibody.
Immunofluorescence analysis of HeLa cells, using Collagen V alpha 3 antibody.
Background:
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Synonyms:
CO5A3; collagen alpha 3;
UniProt Protein Function:
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
UniProt Protein Details:
NCBI Summary:
This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]
