The COL4A6 Polyclonal Antibody (AB10026) is a high-quality antibody designed for researchers studying the collagen type IV alpha 6 chain (COL4A6), a crucial component of basement membranes. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By targeting the COL4A6 protein, this antibody enables precise detection and analysis in a variety of cell types.COL4A6, a key player in basement membrane structure, contributes to tissue integrity and function in various organs, including the kidneys and skin. Dysregulation of COL4A6 has been linked to diseases such as Alport syndrome and certain cancers, highlighting its importance in pathology.
Further research into the role of COL4A6 in these conditions could lead to potential therapeutic interventions aimed at restoring basement membrane integrity and function.With its high specificity and sensitivity, the COL4A6 Polyclonal Antibody is an invaluable tool for investigations in the fields of biology, medicine, and pathology. By facilitating the study of COL4A6 at the molecular level, this antibody opens up new avenues for understanding the role of basement membranes in health and disease.
Product Name:
COL4A6 Polyclonal Antibody
SKU:
CAB22673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant protein of human COL4A6.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
DFNX6; DELXq22.3; CXDELq22.3; COL4A6
Positive Sample:
Rat uterus
Conjugate:
Unconjugated
Cellular Localization:
endoplasmic reticulum lumen, extracellular region, extracellular space
Calculated MW:
164kDa
Observed MW:
170-185kDa
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1288
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Rat uterus, using COL4A6 antibody (CAB22673) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
