Anti-COL4A5 Antibody (CAB9809)
- SKU:
- CAB9809
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-COL4A5 Antibody (CAB9809)
The COL4A5 Polyclonal Antibody (CAB9809) is a valuable tool for researchers studying type IV collagen, a major component of basement membranes in various tissues. This antibody, raised in rabbits, specifically targets the COL4A5 protein and is highly reactive with human samples. Validated for use in Western blot applications, it enables precise detection and analysis of COL4A5 in different cell types.Type IV collagen is essential for the structural integrity of basement membranes and plays a crucial role in cell adhesion, migration, and differentiation. Dysregulation of COL4A5 expression has been associated with various diseases, including Alport syndrome, a genetic disorder affecting the kidneys and eyes.
The ability to accurately assess COL4A5 levels in biological samples is vital for understanding its role in disease pathology and developing targeted therapies.By using the COL4A5 Polyclonal Antibody (CAB9809), researchers can advance their understanding of type IV collagen biology and its implications in health and disease. This antibody is a valuable tool for investigations in nephrology, ophthalmology, and other fields related to extracellular matrix biology.
| Antibody Name: | Anti-COL4A5 Antibody |
| Antibody SKU: | CAB9809 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 22Rv1, NCI-H460, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SSVA HGFL ITRH SQTT DAPQ CPQG TLQV YEGF SLLY VQGN KRAH GQDL GTAG SCLR RFST MPFM FCNI NNVC NFAS RNDY SYWL STPE PMPM SMQP LKGQ SIQP FISR CAVC EAPA VVIA VHSQ TIQI PHCP QGWD SLWI GYSF MMHT SAGA EGSG QALA SPGS CLEE FRSA PFIE CHGR GTCN YYAN SYSF WLAT VDVS DMFS KPQS ETLK AGDL RTRI SRCQ VCMK RT |
| Gene ID: | 1287 |
| Uniprot: | P29400 |
| Cellular Location: | Secreted, basement membrane, extracellular matrix, extracellular space |
| Calculated MW: | 161kDa |
| Observed MW: | 161kDa |
| Synonyms: | COL4A5, ASLN, ATS, CA54 |
| Background: | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. |
| UniProt Protein Function: | COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Extracellular matrix; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: Xq22 Cellular Component: endoplasmic reticulum lumen; extracellular region Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis Disease: Alport Syndrome, X-linked |
| UniProt Code: | P29400 |
| NCBI GenInfo Identifier: | 461675 |
| NCBI Gene ID: | 1287 |
| NCBI Accession: | P29400.2 |
| UniProt Secondary Accession: | P29400,Q16006, Q16126, Q6LD84, Q7Z700, Q9NUB7, |
| Molecular Weight: | 161,632 Da |
| NCBI Full Name: | Collagen alpha-5(IV) chain |
| UniProt Protein Name: | Collagen alpha-5(IV) chain |
| Protein Family: | Collagen |
| UniProt Gene Name: | COL4A5Â Â |
| UniProt Entry Name: | CO4A5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COL4A5 antibody (CAB9809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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