The COL4A4 Polyclonal Antibody is a crucial tool for researchers studying collagen type IV alpha 4 (COL4A4), a key component of basement membranes that plays a pivotal role in maintaining tissue structure and function. This antibody, produced in rabbits, is highly specific to human COL4A4 and has been rigorously validated for use in Western blot applications.COL4A4 is vital for kidney function and mutations in the COL4A4 gene have been linked to kidney diseases, including Alport syndrome. By targeting COL4A4, researchers can investigate the molecular mechanisms underlying these diseases and explore potential therapeutic interventions.
This antibody enables precise detection and quantification of COL4A4 in various cell types and tissue samples, making it an invaluable tool for studies in nephrology, molecular biology, and genetic disorders. By gaining a deeper understanding of the role of COL4A4 in disease pathology, researchers can pave the way for new diagnostic methods and targeted treatments in the future.
Product Name:
COL4A4 Polyclonal Antibody
SKU:
CAB22870
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human COL4A4.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
BFH; ATS2; BFH1; CA44; COL4A4
Positive Sample:
293F
Conjugate:
Unconjugated
Calculated MW:
164kDa
Observed MW:
160kDa
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR.
Purification Method:
Affinity purification
Gene ID:
1286
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of 293F, using COL4A4 antibody (CAB22870) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
