The COL4A3 Polyclonal Antibody (CAB16359) is a valuable tool for researchers studying Collagen IV alpha-3, a key component of basement membranes in various tissues. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the COL4A3 protein, enabling precise detection and analysis in different cell types.Collagen IV alpha-3 is essential for maintaining the structural integrity of basement membranes and plays a crucial role in cell adhesion, migration, and tissue organization.
Research on COL4A3 is important in understanding diseases such as Alport syndrome, a hereditary kidney disorder characterized by mutations in this protein. By studying the function and regulation of COL4A3, researchers can gain insights into its role in disease pathogenesis and potential therapeutic strategies.
Product Name:
COL4A3 Rabbit Polyclonal Antibody
SKU:
CAB16359
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1555-1669 of human COL4A3 (NP_000082.2).
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Purification Method:
Affinity purification
Gene ID:
1285
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse kidney, using COL4A3 antibody (CAB16359) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
