COL4A3 Rabbit Polyclonal Antibody (CAB16359)
- SKU:
- CAB16359
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | COL4A3 Rabbit Polyclonal Antibody |
SKU: | CAB16359 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1555-1669 of human COL4A3 (NP_000082.2). |
Sequence: | AIAI AVHS QTTD IPPC PHGW ISLW KGFS FIMF TSAG SEGT GQAL ASPG SCLE EFRA SPFL ECHG RGTC NYYS NSYS FWLA SLNP ERMF RKPI PSTV KAGE LEKI ISRC QVCM KKR |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
Synonyms: | ATS2; ATS3; BFH2; COL4A3 |
Positive Sample: | Mouse kidney |
Conjugate: | Unconjugated |
Cellular Localization: | Secreted, basement membrane, extracellular matrix, extracellular space. |
Calculated MW: | 162kDa |
Observed MW: | 162kDa/26kDa |
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Purification Method: | Affinity purification |
Gene ID: | 1285 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |