The COL2A1 Polyclonal Antibody (CAB1560) is a valuable tool for researchers studying the COL2A1 protein, which is essential for the structure and function of collagen type II, a key component of cartilage. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in various applications, including Western blotting.COL2A1 is involved in the formation of collagen fibrils in cartilage and other connective tissues, making it a critical target for research in musculoskeletal disorders like osteoarthritis and genetic diseases like chondrodysplasias.
By detecting and analyzing COL2A1 protein levels, researchers can gain valuable insights into the role of collagen type II in cartilage maintenance and repair, providing valuable information for therapeutic development in orthopedics and regenerative medicine.
Product Name:
COL2A1 Rabbit Polyclonal Antibody
SKU:
CAB1560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1220-1282 of human COL2A1 (NP_001835.3).
Sequence:
MSAF AGLG PREK GPDP LQYM RADQ AAGG LRQH DAEV DATL KSLN NQIE SIRS PEGS RKNP ART
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Purification Method:
Affinity purification
Gene ID:
1280
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Rabbit pAb (CAB1560) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
