The Col10a1 Polyclonal Antibody is a valuable tool for researchers studying the COL10A1 protein, which is a key component of type X collagen found in cartilage. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the COL10A1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential asset for studies in musculoskeletal biology and cartilage development.
COL10A1 is crucial for the proper formation and maintenance of cartilage tissue, playing a significant role in skeletal development and bone growth. Dysregulation of COL10A1 expression has been implicated in various musculoskeletal disorders, making it a promising target for research into conditions such as osteoarthritis, skeletal dysplasias, and chondrodysplasias. Understanding the function and regulation of COL10A1 is vital for developing potential therapeutic interventions for these debilitating conditions.
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
Purification Method:
Affinity purification
Gene ID:
1300
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of HT-1080, using COL10A1 Rabbit pAb (CAB23884) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
