The Coilin Rabbit Monoclonal Antibody (CAB3483) is a highly specific antibody designed for research involving the protein coilin, which plays a crucial role in the formation of Cajal bodies within the cell nucleus. This antibody is ideal for studies in cell biology, specifically focusing on nuclear organization and RNA processing.Coilin is a key component of Cajal bodies, specialized sub-nuclear structures involved in various cellular processes such as RNA transcription, splicing, and telomere maintenance. Dysfunction of coilin has been implicated in diseases like spinal muscular atrophy and cancer, making it an important target for research in these areas.
This monoclonal antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. Its specificity to coilin allows for precise detection and analysis of coilin expression in various cell types, making it a valuable tool for researchers studying nuclear organization, RNA processing, and related diseases.
Product Name:
Coilin Rabbit Monoclonal Antibody
SKU:
CAB3483
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Coilin (P38432).
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
Purification Method:
Affinity purification
Gene ID:
8161
Clone Number:
ARC0785
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Coilin Rabbit mAb (CAB3483) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
