The Coilin Polyclonal Antibody (CAB6428) is a valuable tool for researchers studying the protein coilin, which plays a key role in the formation of Cajal bodies in the nucleus of cells. Cajal bodies are important subnuclear structures involved in RNA processing and ribonucleoprotein biogenesis.Raised in rabbits, this antibody is highly specific and reactive to human samples, making it ideal for various research applications. It has been validated for use in Western blotting, immunofluorescence, and immunohistochemistry, allowing for the detection and analysis of coilin in different cell types and tissues.
Coilin is known to interact with a variety of proteins involved in RNA metabolism, including spliceosomal components and small nuclear ribonucleoproteins. Dysregulation of coilin has been linked to neurodegenerative disorders and cancer, making it a promising target for therapeutic interventions.By utilizing the Coilin Polyclonal Antibody (CAB6428), researchers can further elucidate the function of coilin in cellular processes and potentially uncover novel insights into the pathogenesis of diseases associated with Cajal body dysfunction.
Product Name:
Coilin Rabbit Polyclonal Antibody
SKU:
CAB6428
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 297-576 of human Coilin (NP_004636.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CLN80; p80-coilin; Coilin
Positive Sample:
MCF7,HepG2,293T,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Cajal body, Nucleus.
Calculated MW:
63kDa
Observed MW:
80kDa
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
Purification Method:
Affinity purification
Gene ID:
8161
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Coilin Rabbit pAb (CAB6428) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
