The CNFN Antibody (PACO58717) is a high-quality polyclonal antibody designed for research involving CNFN, a protein known for its role in cell adhesion and migration. This antibody, raised in rabbits, is ideal for studies focusing on cell biology, cancer metastasis, and tissue development. It has been validated for use in various applications, including immunofluorescence and flow cytometry.CNFN, also known as cingulin-like protein, is crucial for maintaining cell-cell adhesion and proper tissue organization. Its involvement in cell migration processes makes it a key player in cancer metastasis and tissue regeneration.
By using this antibody in your research, you can effectively detect and analyze CNFN expression in different cell types, gaining valuable insights into its function and potential therapeutic targets.Researchers studying cell biology, cancer biology, and tissue development will find the CNFN Antibody (PACO58717) to be a valuable tool in their experiments. Its specificity and sensitivity make it a reliable option for accurately pinpointing CNFN in various biological samples, ultimately advancing our understanding of cell adhesion mechanisms and disease progression.
IHC image of PACO58717 diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO58717 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Synonyms:
Cornifelin, CNFN
UniProt Protein Function:
CNFN: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the cornifelin family.Chromosomal Location of Human Ortholog: 19q13.2Cellular Component: cytoplasm; cornified envelopeBiological Process: keratinization
