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CM (Ceftiofur Metabolite) ELISA Kit (FSES0064)

SKU:
FSES0064
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
€499
Frequently bought together:

Description

Technical Manual

CM (Ceftiofur Metabolite) ELISA Kit

Kit Name: CM (Ceftiofur Metabolite) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Ceftiofur Metabolite (CM) in samples, such as tissues, egg, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, CM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of CM. The concentration of CM in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 15 ppb (ng/mL)
Reaction Mode: 25°C,30 min~15min
Detection Limit: Tissue, Eggs---200 ppb; Raw milk---100 ppb
Cross Reactivity:

Ceftiofur Metabolite

100%

Ceftiofur

475%

Cefotaxime

250%

Cefquinome

550%
Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA MicrotiterPlate 96 wells
Standard Liquid 1.5 mL each (0 ppb, 15 ppb, 45 ppb, 135 ppb, 405 ppb, 1215 ppb)
Antibody working Solution 7 mL
HRP Conjugate 7 mL
Derivatization Reagent A 2 g
Derivatization Reagent B 5 g
Substrate Reagent A 7 mL
Substrate Reagent B 7 mL
Stop Solution 7 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Oscillators, Centrifuge, Balance (sensibility 0.01g), Water bath.
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: NaOH, KH2PO4,Na2B4O7 10H2O, KCl.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.

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