Clathrin heavy chain Rabbit Polyclonal Antibody (CAB7886)
The CLTC Polyclonal Antibody (CAB7886) is a valuable tool for researchers studying the dynamics of clathrin-mediated endocytosis. Clathrin is a key protein involved in the formation of coated vesicles, essential for the uptake of nutrients and signaling molecules into cells. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot and immunofluorescence applications.By binding to the CLTC protein, this antibody enables researchers to visualize and quantify clathrin-coated vesicles in various cell types, providing insights into the mechanisms of endocytosis.
Understanding the regulation of clathrin-mediated endocytosis is important for unraveling the complexities of cellular trafficking and signaling, with implications for drug delivery, synaptic transmission, and receptor internalization studies.Overall, the CLTC Polyclonal Antibody (CAB7886) is a reliable tool for investigating the role of clathrin in cellular processes, offering valuable insights into the mechanisms underlying vesicle formation and intracellular transport. Its versatility and specificity make it a valuable addition to any research program focused on membrane dynamics and cellular trafficking.
Product Name:
Clathrin heavy chain Rabbit Polyclonal Antibody
SKU:
CAB7886
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Not found
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1550-1650 of human Clathrin heavy chain (NP_004850.1)
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Purification Method:
Affinity purification
Gene ID:
1213
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Clathrin heavy chain antibody (CAB7886) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
