The Clock Polyclonal Antibody (CAB5633) is a vital tool for researchers studying the Clock protein, a key regulator of circadian rhythms and biological clock functions. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been extensively validated for use in Western blot applications. By binding to the Clock protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in neuroscience, chronobiology, and metabolism research.The Clock protein plays a crucial role in regulating the body's internal clock and coordinating various physiological processes, including sleep-wake cycles, metabolism, and hormone production.
Dysregulation of the circadian clock has been associated with a variety of health conditions, such as sleep disorders, metabolic disorders, and mood disorders. By studying the activity of the Clock protein, researchers can gain valuable insights into the mechanisms underlying these disorders and develop targeted therapies to restore circadian rhythm balance.
Product Name:
CLOCK Rabbit Polyclonal Antibody
SKU:
CAB5633
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-600 of human CLOCK (NP_004889.1).
The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9575
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLOCK Rabbit pAb (CAB5633) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
