The CLK4 Polyclonal Antibody (PAC021957) is a valuable tool for researchers studying the cell cycle and signal transduction pathways involving the CDC2-like kinases (CLK) proteins. This antibody, raised in rabbits, has high specificity and sensitivity for CLK4 protein in human samples, making it suitable for Western blot applications.CLK4 is a member of the CLK family of kinases that play important roles in regulating pre-mRNA splicing and modulating gene expression. Dysregulation of CLK proteins has been linked to various diseases, including cancer and neurological disorders.
The ability to specifically target and detect CLK4 with this antibody allows for in-depth studies of its function and potential therapeutic implications in these diseases.By enabling the detection and analysis of CLK4 protein in different cell types and tissues, this antibody serves as a valuable tool for furthering our understanding of the molecular mechanisms underlying disease pathogenesis. Researchers in the field of cell biology, molecular biology, and cancer research will find the CLK4 Polyclonal Antibody essential for their experiments and studies.
Antibody Name:
CLK4 Antibody (PACO21957)
Antibody SKU:
PACO21957
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human CLK4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HT-29 cells, using CLK4 antibody.
Background:
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. McNally T., Protein Sci. 12:1562-1566(2003).
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. [provided by RefSeq, Jul 2008]
