The CLEC9A Monoclonal Antibody (PAC046642) is a valuable tool for researchers studying CLEC9A, a C-type lectin receptor expressed on dendritic cells that plays a crucial role in immune responses. This antibody, produced using PacBio technology, exhibits high specificity and sensitivity for detecting CLEC9A in human samples. Validated for use in various applications including immunofluorescence and flow cytometry, it allows for precise identification and analysis of CLEC9A in different cell populations.
CLEC9A is involved in the recognition and uptake of pathogens by dendritic cells, leading to the activation of immune responses. Its importance in antigen presentation and T cell activation makes it a promising target for studying host-pathogen interactions and vaccine development. By understanding the function and regulation of CLEC9A, researchers can gain insights into immune system dynamics and potentially identify new therapeutic strategies for infectious diseases and immune disorders.
Western blot. All lanes: CLEC9A antibody IgG at 8µg/ml + Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa..
Immunofluorescent analysis of MCF-7 cells using PACO46642 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Synonyms:
C-type lectin domain family 9 member A, CLEC9A
UniProt Protein Function:
CLEC9A: Functions as an endocytic receptor on a small subset of myeloid cells and induces intracellular signaling via spleen tyrosine kinase (Syk). Acts as a receptor for necrotic cells and can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.Protein type: Membrane protein, integral; Receptor, misc.Chromosomal Location of Human Ortholog: 12p13.2Cellular Component: cell surfaceBiological Process: positive regulation of cytokine secretion; receptor-mediated endocytosis
UniProt Protein Details:
NCBI Summary:
CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]
