The Cleaved PARP (Asp214) Polyclonal Antibody is a highly specific tool designed for research involving the cleavage of PARP (poly (ADP-ribose) polymerase) at Asp214, a key event in apoptosis. This antibody, derived from rabbit serum, is ideal for detecting and analyzing the cleavage of PARP in various cell types, making it essential for studies in cell biology, cancer research, and drug development.PARP is a critical enzyme involved in DNA repair and cell survival, and its cleavage at Asp214 by caspases during apoptosis is a hallmark of programmed cell death. Understanding the regulation and dynamics of PARP cleavage is essential for unraveling the mechanisms underlying cell death and survival pathways.
This antibody is validated for use in Western blot applications, offering high sensitivity and specificity in detecting the cleaved form of PARP at Asp214.Furthermore, the Cleaved PARP (Asp214) Polyclonal Antibody can provide valuable insights into the effectiveness of cancer therapies that induce apoptosis, as well as potential targets for novel therapeutic approaches aimed at manipulating cell death pathways. By facilitating the detection and analysis of cleaved PARP at Asp214, this antibody serves as a valuable tool for researchers investigating apoptosis, cancer biology, and drug discovery.
Product Name:
Cleaved PARP (Asp214) Polyclonal Antibody
SKU:
CAB22535
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 201-300 of Mouse PARP (Asp214) (NP_031441.2).
Enables NAD+ ADP-ribosyltransferase activity; chromatin binding activity; and protein ADP-ribosylase activity. Involved in negative regulation of telomere maintenance via telomere lengthening; positive regulation of single strand break repair; and voluntary musculoskeletal movement. Acts upstream of or within several processes, including DNA metabolic process; behavioral response to cocaine; and protein poly-ADP-ribosylation. Located in cytoplasm; nucleolus; and nucleoplasm. Part of protein-containing complex. Is expressed in several structures, including Peyer's patch; brain; gut; immune system; and retina. Human ortholog(s) of this gene implicated in several diseases, including arthritis; bacterial meningitis; neurodegenerative disease (multiple); stomach carcinoma; and type 2 diabetes mellitus. Orthologous to human PARP1 (poly(ADP-ribose) polymerase 1).
Purification Method:
Affinity purification
Gene ID:
11545
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Cleaved PARP (Asp214) Rabbit pAb (CAB22535) at 1:400 dilution.NIH/3T3 cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
