The Lamin A/C Polyclonal Antibody (CAB0249) is a key tool for researchers studying the Lamin A/C protein, a structural component of the nuclear lamina involved in maintaining nuclear shape and stability. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Lamin A/C, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential tool for investigations in cell biology and developmental research.
Lamin A/C is known for its crucial roles in nuclear architecture, gene regulation, and cell differentiation. Dysregulation of Lamin A/C has been linked to various genetic disorders, including muscular dystrophy and progeria, highlighting the importance of studying this protein in both normal and disease states. By utilizing the Lamin A/C Polyclonal Antibody, researchers can gain valuable insights into the functions and dynamics of this essential nuclear protein, potentially leading to new discoveries in the fields of molecular biology and genetics.
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Purification Method:
Affinity purification
Gene ID:
4000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Lamin A/C Rabbit pAb (CAB0249) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
