Cleaved Gasdermin B Polyclonal Antibody (CAB23046)
The Cleaved Gasdermin B Polyclonal Antibody (CAB23046) is a valuable tool for researchers studying gasdermins, key players in pyroptosis, a form of programmed cell death. This antibody, generated in rabbits, is highly specific for cleaved Gasdermin B and is validated for use in various experimental techniques, including Western blot analysis.Gasdermin B is involved in the formation of pores in cell membranes during pyroptosis, leading to cell lysis and release of pro-inflammatory cytokines.
Understanding the cleavage and activation of Gasdermin B is crucial for elucidating the mechanisms driving this inflammatory cell death pathway. The CAB23046 antibody enables researchers to detect and analyze cleaved Gasdermin B in different cell types, providing insights into its role in inflammation, infection, and other pathological conditions. This antibody is a valuable tool for studies in immunology, cell biology, and disease research.
Product Name:
Cleaved Gasdermin B Polyclonal Antibody
SKU:
CAB23046
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human Cleaved Gasdermin B.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
GSDML; PP4052; GSDMB-1; PRO2521; Cleaved Gasdermin B
Positive Sample:
HT-29
Conjugate:
Unconjugated
Calculated MW:
18kDa/45kDa/46kDa/47kDa
Observed MW:
16kDa
This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.
Purification Method:
Affinity purification
Gene ID:
55876
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29+NK-92, using Cleaved Gasdermin B Rabbit pAb (CAB23046) at 1:400 dilution.HT-29 and NK-92 cells were treated by IFNγ (50ng/ml) for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
