The Cleaved Caspase-7 Monoclonal Antibody is a valuable tool for researchers studying apoptosis, particularly the role of caspase-7 in programmed cell death. This antibody, developed and validated for use in various applications, including Western blot and immunohistochemistry, specifically targets the cleaved form of caspase-7, allowing for precise detection and analysis in cell samples.Caspase-7 is a key player in the execution phase of apoptosis, where it cleaves specific cellular proteins to initiate cell death processes. Understanding the activity and regulation of caspase-7 is important for unraveling the mechanisms underlying cell death pathways, with implications for cancer research, neurodegenerative diseases, and other physiological processes.
By using the Cleaved Caspase-7 Monoclonal Antibody, researchers can gain insights into the dynamics of apoptosis and potential therapeutic targets for diseases characterized by dysregulated cell death mechanisms. Its high specificity and sensitivity make it a valuable asset for studies aiming to elucidate the roles of caspase-7 in health and disease.
Product Name:
Cleaved Caspase-7 Monoclonal Antibody
SKU:
CAB23154
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CASP7 (NP_001218.1).
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
840
Clone Number:
ARC58776
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells using Cleaved Caspase-7 Rabbit mAb (CAB23154) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
