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Cleaved Caspase-7 Monoclonal Antibody (CAB23154)

SKU:
CAB23154
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cleaved Caspase-7 Monoclonal Antibody
SKU:CAB23154
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CASP7 (NP_001218.1).
Sequence:CFRS LGFD VIVY NDCS CAKM QDLL KKAS EEDH TNAA CFAC ILLS HGEE NVIY GKDG VTPI KDLT AHFR GDRC KTLL EKPK LFFI QACR GTEL DDGI QADS G
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3; Cleaved Caspase-7
Positive Sample:Jurkat+Etoposide
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:34kDa
Observed MW:22kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification Method:Affinity purification
Gene ID:840
Clone Number:ARC58776
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells using Cleaved Caspase-7 Rabbit mAb (CAB23154) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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