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Cleaved-CASP4/CASP5 (D270/D311) Antibody (PACO05468)

SKU:
PACO05468
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€319

Description

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Cleaved-CASP4/CASP5 (D270/D311) Antibody (PACO05468)

The Cleaved Casp4/Casp5 (D270/D311) Antibody (PAC00548) is a highly specific and sensitive tool for studying the cleavage of caspases 4 and 5 at the D270 and D311 sites, respectively. These caspases play crucial roles in the inflammatory response and pyroptosis, making them key players in immune regulation and cell death pathways. This polyclonal antibody, produced in rabbits, is validated for detection in human samples and is recommended for use in Western blot applications. By targeting the cleavage sites of caspases 4 and 5, researchers can better understand the mechanisms underlying inflammatory processes and pyroptotic cell death.

With its high reactivity and specificity, the Cleaved Casp4/Casp5 (D270/D311) Antibody is an essential tool for studies in immunology, cell biology, and disease research. Its ability to detect cleaved forms of caspases 4 and 5 provides valuable insights into the molecular events that drive inflammation and cell death, offering new avenues for therapeutic intervention in conditions such as inflammatory diseases and cancer.

Antibody Name:Cleaved-CASP4/CASP5 (D270/D311) Antibody (PACO05468)
Antibody SKU:PACO05468
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:40000, WB:1:500-1:2000
Species Reactivity:Human
Immunogen:Synthesized peptide derived from the Internal region of human Caspase-4/5 p20.
Form:Liquid
Storage Buffer:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Synonyms:CASP4; ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TX; CASP5; ICH3; Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY
UniProt Protein Function:CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.22.64; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion

Biological Process: apoptosis; proteolysis; regulation of apoptosis; regulation of inflammatory response

NCBI Summary:This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:P49662
NCBI GenInfo Identifier:1352420
NCBI Gene ID:837
NCBI Accession:P49662.1
UniProt Secondary Accession:P49662,A2NHL8, A2NHM0,
UniProt Related Accession:P49662
Molecular Weight:36,705 Da
NCBI Full Name:Caspase-4
NCBI Synonym Full Names:caspase 4
NCBI Official Symbol:CASP4  
NCBI Official Synonym Symbols:TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II  
NCBI Protein Information:caspase-4
UniProt Protein Name:Caspase-4
UniProt Synonym Protein Names:ICE(rel)-II; Protease ICH-2; Protease TX
Protein Family:Caspase
UniProt Gene Name:CASP4  
UniProt Entry Name:CASP4_HUMAN

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