The CLCNKA Polyclonal Antibody (CAB3792) is specifically designed for research involving the CLCNKA gene, which encodes a kidney-specific chloride channel involved in regulating salt and water balance. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in various research applications including Western blot and immunohistochemistry.The CLCNKA gene plays a crucial role in maintaining electrolyte balance and renal function, making it a key target for studying kidney disorders and hypertension. By detecting the expression of CLCNKA protein, researchers can gain insights into the function and regulation of chloride channels in the kidney, opening up avenues for potential therapeutic interventions in salt-sensitive hypertension and other related conditions.
Overall, the CLCNKA Polyclonal Antibody (CAB3792) is a valuable tool for researchers interested in studying the molecular mechanisms underlying kidney function and water-electrolyte balance, with potential implications for the development of targeted therapies for renal disorders and hypertension.
Product Name:
CLCNKA Rabbit Polyclonal Antibody
SKU:
CAB3792
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 470-644 of human CLCNKA (NP_001244068.1).
This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1187
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLCNKA Rabbit pAb (CAB3792) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
