The CLCN5 Polyclonal Antibody (CAB5707) is a valuable tool for researchers studying the CLCN5 protein, a member of the chloride channel family involved in kidney function and calcium metabolism. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CLCN5 protein, this antibody allows for the detection and analysis of CLCN5 in various cell types, making it an essential reagent for studies in nephrology and metabolic disorders.
CLCN5 is known to play a crucial role in the regulation of chloride ions and pH balance in the kidney, making it a potential target for research into kidney diseases, such as Dent disease and Bartter syndrome. Understanding the function and regulation of CLCN5 can provide insights into the pathogenesis of these disorders and potentially lead to the development of targeted therapies. The CLCN5 Polyclonal Antibody is a valuable tool for researchers looking to further explore the role of the CLCN5 protein in kidney physiology and disease.
Product Name:
CLCN5 Rabbit Polyclonal Antibody
SKU:
CAB5707
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 637-816 of human CLCN5 (NP_001121370.1).
This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CLCN5 antibody (CAB5707) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
