The Claudin-3 Polyclonal Antibody (CAB11650) is a valuable tool for researchers studying Claudin-3, a tight junction protein essential for maintaining epithelial barrier function. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Claudin-3 in human samples, making it suitable for various applications such as Western blotting and immunohistochemistry.Claudin-3 is involved in regulating paracellular permeability and maintaining tissue integrity in various organs, including the kidneys, intestines, and lungs. Dysregulation of Claudin-3 has been linked to various diseases, such as cancer, inflammatory bowel disease, and pulmonary disorders.
By utilizing the Claudin-3 Polyclonal Antibody, researchers can investigate the role of Claudin-3 in disease pathogenesis and potential therapeutic interventions targeting this protein.Overall, the Claudin-3 Polyclonal Antibody is a reliable tool for studying the function and expression of Claudin-3 in physiological and pathological conditions, contributing to advancements in research areas such as epithelial biology, cancer biology, and gastrointestinal health.
Product Name:
CLDN3 Rabbit Polyclonal Antibody
SKU:
CAB11650
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 141-220 of human CLDN3 (NP_001297.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.
Purification Method:
Affinity purification
Gene ID:
1365
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLDN3 Rabbit pAb (CAB11650) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
