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Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB01674
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Reactivity:
Human
Detection Method:
Colorimetric
$719
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Description

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Claudin 3 (Phospho-Tyr219)Colorimetric Cell-Based ELISA Kit

The Claudin-3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit from Assay Genie is a cutting-edge tool for the precise measurement of phosphorylated Claudin-3 levels in cell lysates. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for your research needs.Claudin-3 is a key protein in the tight junctions of epithelial cells, regulating paracellular permeability and cell-cell adhesion. Phosphorylation of Claudin-3 at Tyr219 has been implicated in various diseases, including cancer and inflammatory disorders, making it a valuable target for investigation and potential therapeutic interventions.

With the Assay Genie Claudin-3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit, researchers can delve into the intricate signaling pathways involving Claudin-3 phosphorylation, shedding light on its role in disease progression and paving the way for innovative treatment strategies. Trust Assay Genie for reliable and cutting-edge tools to advance your research efforts.

Product Name:Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA
Product Code:CBCAB01674
ELISA Type:Cell-Based
Target:Claudin 3 (Phospho-Tyr219)
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Claudin 3 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Claudin 3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 3 phosphorylation.

Qualitative determination of Claudin 3 (Phospho-Tyr219) concentration is achieved by an indirect ELISA format. In essence, Claudin 3 (Phospho-Tyr219) is captured by Claudin 3 (Phospho-Tyr219)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 1365, UniProt ID: O15551, OMIM: 602910, Unigene: Hs.647023
Gene Symbol:CLDN3
Sub Type:Phospho
UniProt Protein Function:Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: apicolateral plasma membrane; integral to membrane; integral to plasma membrane; lateral plasma membrane; tight junction

Molecular Function:identical protein binding; structural molecule activity; transmembrane receptor activity

Biological Process: calcium-independent cell-cell adhesion; protein heterooligomerization; protein homooligomerization; response to hypoxia; signal transduction

NCBI Summary:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
UniProt Code:O15551
NCBI GenInfo Identifier:6685273
NCBI Gene ID:1365
NCBI Accession:O15551.1
UniProt Secondary Accession:O15551,Q9Z0G9, Q63400,
UniProt Related Accession:O15551
Molecular Weight:23,319 Da
NCBI Full Name:Claudin-3
NCBI Synonym Full Names:claudin 3
NCBI Official Symbol:CLDN3  
NCBI Official Synonym Symbols:RVP1; HRVP1; C7orf1; CPE-R2; CPETR2  
NCBI Protein Information:claudin-3
UniProt Protein Name:Claudin-3
UniProt Synonym Protein Names:Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1
Protein Family:Claudin
UniProt Gene Name:CLDN3  
UniProt Entry Name:CLD3_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-Claudin 3 (Phospho-Tyr219) Antibody, Anti-Claudin 3 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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