The Claudin-2 Polyclonal Antibody (CAB6560) is a valuable tool for research involving Claudin-2, an integral membrane protein involved in tight junctions and paracellular ion transport. This antibody, developed in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the Claudin-2 protein, it allows for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and epithelial barrier function.Claudin-2 is known for its role in regulating paracellular permeability and ion selectivity in epithelial cells, making it a key player in maintaining tissue integrity and barrier function.
Dysregulation of Claudin-2 has been implicated in a variety of diseases, including gastrointestinal disorders and certain types of cancer. Research on Claudin-2 using this antibody can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.
Product Name:
CLDN2 Rabbit Polyclonal Antibody
SKU:
CAB6560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human CLDN2 (NP_001164566.1).
This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9075
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CLDN2 antibody (CAB6560) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
