Anti-Claudin-10 Antibody (CAB9853)

Antibody Name:	Anti-Claudin-10 Antibody
Antibody SKU:	CAB9853
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CLDN10 (NP_008915.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CLDN10 (NP_008915.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAST ASEI IAFM VSIS GWVL VSST LPTD YWKV STID GTVI TTAT YWAN LWKA CVTD STGV SNCK DFPS MLAL DGYI QACR GLMI AAVS LGFF GSIF ALFG
Gene ID:	9071
Uniprot:	P78369
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, tight junction
Calculated MW:	22kDa/24kDa
Observed MW:	26kDa

Synonyms:

CLDN10, CPETRL3, OSP-L

Background:

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma. Six alternatively spliced transcript variants encoding different isoforms have been reported, but the transcript sequences of some variants are not determined.

UniProt Protein Function:	Claudin-10: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 13q32.1 Cellular Component: tight junction Molecular Function:identical protein binding Biological Process: calcium-independent cell-cell adhesion; cell adhesion
NCBI Summary:	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma. Six alternatively spliced transcript variants encoding different isoforms have been reported, but the transcript sequences of some variants are not determined.[provided by RefSeq, Jun 2010]
UniProt Code:	P78369
NCBI GenInfo Identifier:	6685311
NCBI Gene ID:	9071
NCBI Accession:	P78369.2
UniProt Secondary Accession:	P78369,Q6IBF9, Q96N78,
UniProt Related Accession:	P78369
Molecular Weight:	24kDa
NCBI Full Name:	Claudin-10
NCBI Synonym Full Names:	claudin 10
NCBI Official Symbol:	CLDN10
NCBI Official Synonym Symbols:	OSP-L; CPETRL3
NCBI Protein Information:	claudin-10
UniProt Protein Name:	Claudin-10
UniProt Synonym Protein Names:	OSP-like protein
Protein Family:	Claudin
UniProt Gene Name:	CLDN10

Anti-Claudin-10 Polyclonal Antibody (CAB9853)

Western blot analysis of extracts of rat lung, using CLDN10 antibody (CAB9853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.

Antibodies	ELISA Kits
CLDN10 Antibody (PACO05679)	Claudin 10 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)