The Claudin-1 Polyclonal Antibody (CAB2196) is a valuable tool for researchers studying Claudin-1, a key protein involved in the formation of tight junctions in epithelial cells. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it ideal for use in a variety of research applications, including Western blotting.Claudin-1 is crucial for the maintenance of epithelial barrier function, playing a critical role in regulating the movement of ions and macromolecules across cell layers. Dysregulation of Claudin-1 has been implicated in various diseases, including cancer and inflammatory disorders, making it a promising target for therapeutic interventions.
By using the Claudin-1 Polyclonal Antibody, researchers can accurately detect and analyze Claudin-1 expression in different cell types, aiding in the understanding of its role in health and disease. This antibody is a valuable tool for studies in epithelial biology, cancer research, and drug development efforts targeting Claudin-1 as a therapeutic target.
Product Name:
Claudin 1 Rabbit Polyclonal Antibody
SKU:
CAB2196
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 137-211 of human Claudin 1 (NP_066924.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Purification Method:
Affinity purification
Gene ID:
9076
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of A-431, using Claudin 1 Rabbit pAb (CAB2196) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
