The Claudin-1 Polyclonal Antibody (CAB21949) is a valuable tool for researchers studying Claudin-1, a key protein involved in tight junctions and cell barrier function. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the Claudin-1 protein, this antibody enables researchers to detect and analyze Claudin-1 expression in a variety of cell types, making it ideal for studies in molecular biology, cell biology, and cancer research.
Claudin-1 plays a crucial role in maintaining the integrity of epithelial barriers and regulating the permeability of tight junctions. Dysregulation of Claudin-1 has been implicated in various diseases, including cancer and inflammatory disorders. By understanding the function and expression of Claudin-1, researchers can gain insights into disease mechanisms and potential therapeutic targets. The Claudin-1 Polyclonal Antibody provides a reliable tool for investigating the role of Claudin-1 in health and disease.
Product Name:
Claudin 1 Polyclonal Antibody
SKU:
CAB21949
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 137-211 of human Claudin 1 (NP_066924.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Purification Method:
Affinity purification
Gene ID:
9076
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat kidney, using Claudin 1 antibody (CAB21949) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
