The Claudin-1 Polyclonal Antibody (CAB21770) is a valuable tool for researchers studying the protein Claudin-1, an integral membrane protein involved in the tight junctions of epithelial and endothelial cells. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Claudin-1, this antibody allows for the precise detection and analysis of the protein in various cell types, making it a crucial component for studies in the fields of cell biology, cancer research, and epithelial barrier function.Claudin-1 plays a crucial role in the maintenance of tissue barriers and the regulation of paracellular transport, making it a key player in physiological processes such as epithelial cell polarity and the prevention of leaky barriers.
Dysregulation of Claudin-1 has been implicated in various diseases, including cancer and inflammatory disorders, highlighting the importance of understanding its function and regulation. By utilizing the Claudin-1 Polyclonal Antibody, researchers can gain valuable insights into the role of Claudin-1 in health and disease, paving the way for potential therapeutic interventions targeting this protein.
Product Name:
Claudin 1 Polyclonal Antibody
SKU:
CAB21770
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 111-211 of human Claudin 1 (NP_066924.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Purification Method:
Affinity purification
Gene ID:
9076
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Claudin 1 Rabbit pAb (CAB21770) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
