The Claudin-1 Monoclonal Antibody (CAB21971) is a vital tool for researchers studying the expression and function of the Claudin-1 protein, a key component of tight junctions in epithelial cells. This monoclonal antibody is specifically designed to target Claudin-1 in human samples, making it an ideal choice for experiments involving cell-cell adhesion, barrier function, and epithelial permeability.Raised in mice, this antibody has been rigorously tested and validated for use in various applications, including immunofluorescence, immunohistochemistry, and flow cytometry. Its high specificity and sensitivity enable precise detection and localization of Claudin-1, allowing researchers to investigate its role in epithelial biology, cancer metastasis, and inflammatory responses.
The Claudin-1 Monoclonal Antibody holds great potential for advancing our understanding of tight junction dynamics and cell signaling pathways in health and disease. By targeting Claudin-1 with this antibody, researchers can unravel the complexities of epithelial barrier function and explore novel therapeutic strategies for conditions such as inflammatory bowel disease, viral infections, and cancer progression.
Product Name:
Claudin 1 Monoclonal Antibody
SKU:
CAB21971
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 112-211 of human Claudin 1 (NP_066924.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Purification Method:
Affinity purification
Gene ID:
9076
Clone Number:
ARC54475
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (CAB21971) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
