The CKMT1B Polyclonal Antibody (CAB3046) is a valuable tool for researchers studying CKMT1B, a key enzyme involved in cellular energy metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CKMT1B protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in biochemistry and mitochondrial function.CKMT1B, also known as mitochondrial creatine kinase, plays a crucial role in maintaining energy balance in cells by facilitating the transfer of high-energy phosphate groups. Dysfunction of CKMT1B has been linked to various diseases, including metabolic disorders and heart failure, highlighting its importance in cellular health.
Research into the function and regulation of CKMT1B is essential for understanding these pathologies and developing targeted therapeutic interventions to restore energy metabolism.Overall, the CKMT1B Polyclonal Antibody (CAB3046) offers researchers a reliable tool for investigating the role of CKMT1B in cellular energy metabolism and its implications for human health and disease. By enabling accurate detection and analysis of CKMT1B in research samples, this antibody contributes to advancing our understanding of cellular bioenergetics and potential therapeutic strategies for related disorders.
Product Name:
CKMT1B Rabbit Polyclonal Antibody
SKU:
CAB3046
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.
Purification Method:
Affinity purification
Gene ID:
1159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CKMT1B Rabbit pAb (CAB3046) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
