The CKM Polyclonal Antibody (CAB2024) is a valuable tool for researchers studying creatine kinase M (CKM), an enzyme involved in cellular energy metabolism and muscle function. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.CKM is primarily found in skeletal muscle, where it plays a key role in the regeneration of ATP during intense exercise. Dysregulation of CKM has been linked to various muscle disorders and metabolic diseases, making it an important target for research in the fields of exercise physiology, sports science, and muscle biology.
By targeting the CKM protein, this antibody allows for the detection and analysis of CKM expression in different cell types and tissues. Its high specificity and sensitivity make it an ideal tool for investigating the role of CKM in physiological processes and disease states.Overall, the CKM Polyclonal Antibody is a valuable resource for researchers looking to further understand the function and regulation of CKM in health and disease. Its versatility and reliability make it a valuable addition to any laboratory conducting research in the fields of muscle biology and metabolism.
Product Name:
CKM Rabbit Polyclonal Antibody
SKU:
CAB2024
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 3-381 of human CKM (NP_001815.2).
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Purification Method:
Affinity purification
Gene ID:
1158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse skeletal muscle, using CKM antibody (CAB2024) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
