The CKAP5 Polyclonal Antibody (CAB8532) is a valuable tool for researchers studying CKAP5, a protein involved in cell division and microtubule organization. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CKAP5 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in cell biology and cancer research.
CKAP5, also known as cytoskeleton-associated protein 5, plays a crucial role in maintaining the structural integrity of cells during division. Dysregulation of CKAP5 has been implicated in various diseases, including cancer, making it a potential therapeutic target. By studying the function of CKAP5, researchers can gain valuable insights into cell division processes and potentially identify new strategies for treating cancer and other related disorders.
Product Name:
CKAP5 Rabbit Polyclonal Antibody
SKU:
CAB8532
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1).
Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
225kDa
Observed MW:
250kDa
This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
9793
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CKAP5 Rabbit pAb (CAB8532) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
