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CISH Rabbit Polyclonal Antibody (CAB14527)

SKU:
CAB14527
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
  • Developmental Biology Anti-CISH Antibody CAB14527
  • Developmental Biology Anti-CISH Antibody CAB14527
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CISH Rabbit Polyclonal Antibody
SKU:CAB14527
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1).
Sequence:TGQR PLWA PSLE LPKP VMQP LPAG AFLE EVAE GTPA QTES EPKV LDPE EDLL CIAK TFSY LRES GWYW GSIT ASEA RQHL QKMP EGTF LVRD STHP SYLF TLSV KTTR GPTN VR
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:CIS; G18; SOCS; CIS-1; BACTS2; CISH
Positive Sample:K-562,Mouse kidney,Rat kidney
Conjugate:Unconjugated
Cellular Localization:cytosol, plasma membrane
Calculated MW:29kDa
Observed MW:37kDa

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:1154
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using CISH Rabbit pAb (CAB14527) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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