The CISD1 Polyclonal Antibody (CAB10317) is a valuable tool for researchers studying CISD1, a mitochondrial protein involved in regulating iron homeostasis and cell survival. This antibody, produced in rabbits, is specifically designed to target CISD1 in human samples and has been validated for use in Western blot applications. By binding to CISD1, this antibody allows for the detection and analysis of the protein in various cell types, making it ideal for studies in mitochondrial biology and iron metabolism.CISD1, also known as CDGSH iron sulfur domain 1, plays a crucial role in maintaining cellular iron levels and protecting cells from oxidative damage.
Dysregulation of CISD1 has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes. Research on CISD1 is important for understanding the mechanisms underlying these conditions and developing potential therapeutic interventions.Overall, the CISD1 Polyclonal Antibody is a reliable tool for investigating the function of CISD1 and its implications in human health and disease. Its high specificity and sensitivity make it a valuable asset for researchers looking to advance our understanding of mitochondrial biology and iron homeostasis.
Product Name:
CISD1 Rabbit Polyclonal Antibody
SKU:
CAB10317
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-108 of human CISD1 (NP_060934.1).
Mitochondrion outer membrane, Single-pass type III membrane protein.
Calculated MW:
12kDa
Observed MW:
16kDa
This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.
Purification Method:
Affinity purification
Gene ID:
55847
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CISD1 antibody (CAB10317) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
