The CHST13 Polyclonal Antibody (PAC023403) is a valuable tool for researchers studying CHST13, a key enzyme involved in heparan sulfate biosynthesis. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the CHST13 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for investigations in glycobiology and cancer biology.CHST13, also known as carbohydrate sulfotransferase 13, plays a crucial role in the modification of heparan sulfate, a vital component of the extracellular matrix involved in cell signaling and adhesion.
Dysregulation of CHST13 has been implicated in various diseases, including cancer and inflammatory disorders. Studying the function of CHST13 is essential for gaining insights into the pathogenesis of these conditions and developing targeted therapies.Overall, the CHST13 Polyclonal Antibody offers researchers a reliable tool for investigating the role of CHST13 in health and disease, paving the way for potential therapeutic advancements in the field of glycobiology.
Synthesized peptide derived from internal of human CHST13.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from K562 cells, using CHST13 antibody.
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CHST13 antibody.
Background:
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan.
Synonyms:
Tumor necrosis factor receptor superfamily member 6B; Decoy receptor for Fas ligand; Decoy receptor 3; DcR3; M68
UniProt Protein Function:
CHST13: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. Belongs to the sulfotransferase 2 family.Protein type: EC 2.8.2.5; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase; Energy Metabolism - sulfurChromosomal Location of Human Ortholog: 3q21.3Cellular Component: Golgi membrane; integral to membraneMolecular Function: chondroitin 4-sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activityBiological Process: carbohydrate biosynthetic process; carbohydrate metabolic process; chondroitin sulfate biosynthetic process; chondroitin sulfate metabolic process; glycosaminoglycan metabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to the C4 hydroxyl of beta-1,4-linked N-acetylgalactosamine (GalNAc) flanked by glucuronic acid residue in chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. [provided by RefSeq, Aug 2011]
