The CHST12 Polyclonal Antibody (CAB17721) is a high-quality research tool designed for studying CHST12, a key protein involved in the biosynthesis of chondroitin sulfate. This antibody, derived from rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.CHST12 plays a critical role in the modification of proteoglycans, which are essential components of the extracellular matrix and play a crucial role in cell signaling, adhesion, and migration. Dysregulation of chondroitin sulfate biosynthesis has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions.
By using the CHST12 Polyclonal Antibody, researchers can effectively detect and analyze the expression of CHST12 in different cell types and tissues, providing valuable insights into its functions and potential therapeutic applications. This antibody is a valuable tool for studies in glycobiology, cancer research, and regenerative medicine.
Product Name:
CHST12 Rabbit Polyclonal Antibody
SKU:
CAB17721
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 193-414 of human CHST12 (NP_061111.1).
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
55501
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using CHST12 Rabbit pAb (CAB17721) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
