Anti-CHST10 Antibody (CAB4307)

Antibody Name:	Anti-CHST10 Antibody
Antibody SKU:	CAB4307
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-250 of human CHST10 (NP_004845.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	BxPC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-250 of human CHST10 (NP_004845.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KDPD VYSA KQEF LFLT TMPE VRKL PEEK HIPE ELKP TGKE LPDS QLVQ PLVY MERL ELIR NVCR DDAL KNLS HTPV SKFV LDRI FVCD KHKI LFCQ TPKV GNTQ WKKV LIVL NGAF SSIE EIPE NVVH DHEK NGLP RLSS FSDA EIQK RLKT YFKF FIVR DPFE RLIS AFKD KFVH NPRF EPWY RHEI APGI IRKY RRNR TETR GIQF EDFV RYLG DPNH R
Gene ID:	9486
Uniprot:	O43529
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:	CHST10, HNK-1ST, HNK1ST
Background:	This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.

UniProt Protein Function:	HNK1ST: Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis. Belongs to the sulfotransferase 2 family.
UniProt Protein Details:	Protein type:EC 2.8.2.-; Membrane protein, integral; Transferase Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane Molecular Function:sulfotransferase activity; HNK-1 sulfotransferase activity Biological Process: long-term memory; learning; cell adhesion; carbohydrate biosynthetic process
NCBI Summary:	This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011]
UniProt Code:	O43529
NCBI GenInfo Identifier:	61211695
NCBI Gene ID:	9486
NCBI Accession:	O43529.1
UniProt Secondary Accession:	O43529,Q53T18,
UniProt Related Accession:	O43529
Molecular Weight:	356
NCBI Full Name:	Carbohydrate sulfotransferase 10
NCBI Synonym Full Names:	carbohydrate sulfotransferase 10
NCBI Official Symbol:	CHST10
NCBI Official Synonym Symbols:	HNK1ST; HNK-1ST
NCBI Protein Information:	carbohydrate sulfotransferase 10; huHNK-1ST; HNK-1 sulfotransferase
UniProt Protein Name:	Carbohydrate sulfotransferase 10
UniProt Synonym Protein Names:	HNK-1 sulfotransferase; HNK-1ST; HNK1ST; HuHNK-1ST
Protein Family:	Carbohydrate sulfotransferase
UniProt Gene Name:	CHST10
UniProt Entry Name:	CHSTA_HUMAN

Western blot analysis of extracts of BxPC-3 cells, using CHST10 antibody (CAB4307) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

CHST10 Antibody (PACO00921)

CHST10 Antibody (PACO08425)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)