The CHRNE Polyclonal Antibody (CAB10057) is a specialized tool for researchers studying CHRNE, a key component of the muscle acetylcholine receptor. Produced in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CHRNE protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable resource for investigations in the fields of neurobiology and muscle physiology.
CHRNE plays a crucial role in neuromuscular transmission and is essential for proper muscle function. Dysregulation of CHRNE has been implicated in various neuromuscular disorders, making it a potential target for therapeutic interventions. By studying the function and expression of CHRNE using this antibody, researchers can gain insights into the mechanisms underlying neuromuscular diseases and potentially develop novel treatment strategies.
Product Name:
CHRNE Rabbit Polyclonal Antibody
SKU:
CAB10057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of human CHRNE (NP_000071.1).
Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome.
Purification Method:
Affinity purification
Gene ID:
1145
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHRNE antibody (CAB10057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
