The CHRNA5 Polyclonal Antibody (CAB10467) is a valuable tool for researchers studying the CHRNA5 gene, which encodes a subunit of the nicotinic acetylcholine receptor. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By targeting the CHRNA5 protein, this antibody allows for the accurate detection and analysis of this receptor subunit in various cell types, making it particularly useful for studies in neuroscience and addiction research.
The nicotinic acetylcholine receptor, to which CHRNA5 belongs, plays a crucial role in mediating synaptic transmission in the nervous system. Mutations in the CHRNA5 gene have been associated with various neurological and psychiatric disorders, including nicotine dependence and schizophrenia. By investigating the function and regulation of CHRNA5, researchers can gain insights into the pathophysiology of these disorders and potentially identify new therapeutic targets for intervention.
Product Name:
CHRND Rabbit Polyclonal Antibody
SKU:
CAB10467
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-245 of human CHRND (NP_000742.1).
The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL), congenital myasthenic syndrome slow-channel type (SCCMS), and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1144
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CHRND Rabbit pAb (CAB10467) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
