The CHRNA3 Polyclonal Antibody (CAB1674) is a valuable tool for researchers studying the CHRNA3 gene, which encodes a subunit of the nicotinic acetylcholine receptor. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the CHRNA3 protein, this antibody allows for the detection and analysis of CHRNA3 in a variety of cell types, making it ideal for studies in neuroscience, addiction research, and pharmacology.The nicotinic acetylcholine receptor, to which CHRNA3 belongs, is known to play a crucial role in mediating the effects of nicotine in the brain.
Mutations in the CHRNA3 gene have been associated with various neurological disorders, including addiction and cognitive impairments. Research into the function of CHRNA3 is essential for gaining a deeper understanding of these conditions and may lead to the development of targeted therapies.Overall, the CHRNA3 Polyclonal Antibody (CAB1674) is a valuable tool for researchers investigating the role of nicotinic acetylcholine receptors in the brain and related neurological disorders. Its high reactivity and specificity make it a reliable choice for studies aiming to uncover the mechanisms underlying nicotine addiction and cognitive function.
Product Name:
CHRNA3 Rabbit Polyclonal Antibody
SKU:
CAB1674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 32-240 of human CHRNA3 (NP_000734.2).
This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
1136
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHRNA3 antibody (CAB1674) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
