Chrna1 Antibody (PACO25757)
- SKU:
- PACO25757
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Chrna1 Antibody (PACO25757)
The CHRNA1 Polyclonal Antibody (PAC025757) is specially designed for researchers studying CHRNA1, a protein that plays a key role in muscle contraction through its function as an acetylcholine receptor. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CHRNA1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal tool for investigating muscle function and neuromuscular disorders.CHRNA1, also known as nicotinic acetylcholine receptor alpha 1, is essential for transmitting acetylcholine signals at the neuromuscular junction, ultimately leading to muscle contraction.
Dysregulation of CHRNA1 is associated with conditions such as myasthenia gravis and congenital myasthenic syndromes, highlighting its importance in maintaining muscle function. Research into the role of CHRNA1 can provide valuable insights into these disorders and potentially lead to the development of new treatment strategies targeted at modulating muscle contraction mechanisms.
| Antibody Name: | Chrna1 Antibody (PACO25757) |
| Antibody SKU: | PACO25757 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Mouse, Human |
| Immunogen: | Recombinant Mouse Acetylcholine receptor subunit α protein (21-230AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: Chrna1 antibody at 2µg/ml + Hela whole lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa . |
| Background: | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
| Synonyms: | Acetylcholine receptor subunit alpha, Chrna1, Acra |
| UniProt Protein Function: | nAChRA1: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA1 are a cause of multiple pterygium syndrome lethal type (MUPSL). Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent. The alpha subunit is the main focus for antibody binding in myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs. Defects in CHRNA1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRNA1 are a cause of congenital myasthenic syndrome fast-channel type (FCCMS). FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 1/CHRNA1 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.Chromosomal Location of Human Ortholog: 2 C3|2 43.76 cMCellular Component: cell; cell surface; membrane; neuromuscular junction; nicotinic acetylcholine-gated receptor-channel complex; plasma membraneMolecular Function: acetylcholine binding; acetylcholine receptor activity; acetylcholine-gated cation channel activity; protein bindingBiological Process: cation transport; generation of action potential; muscle maintenance; musculoskeletal movement; neuromuscular junction development; neuromuscular process; neuromuscular synaptic transmission; regulation of membrane potential; response to nicotine; skeletal muscle contraction; skeletal muscle growth; synaptic transmission, cholinergic |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes an alpha subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The alpha subunit plays a role in substrate binding and channel gating. [provided by RefSeq, Nov 2012] |
| UniProt Code: | P04756 |
| NCBI GenInfo Identifier: | 31542391 |
| NCBI Gene ID: | 11435 |
| NCBI Accession: | NP_031415.2 |
| UniProt Secondary Accession: | P04756 |
| UniProt Related Accession: | P04756 |
| Molecular Weight: | 26.5kD |
| NCBI Full Name: | acetylcholine receptor subunit alpha |
| NCBI Synonym Full Names: | cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) |
| NCBI Official Symbol: | Chrna1Â Â |
| NCBI Official Synonym Symbols: | Acra; Achr-1; AI385656; AI608266Â Â |
| NCBI Protein Information: | acetylcholine receptor subunit alpha |
| UniProt Protein Name: | Acetylcholine receptor subunit alpha |
| UniProt Synonym Protein Names: | |
| Protein Family: | Acetylcholine receptor |
| UniProt Gene Name: | Chrna1Â Â |
| UniProt Entry Name: |
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